Effects of vaccination on malaria-induced suppression of SULT2a and CYP7a1. (A) Analysis of hepatic SULT2a1 and CYP7a1 expression on the indicated days of P. chabaudi malaria in vaccinated (triangles) and non-vaccinated (circles) mice by real-time RT-PCR. Signals were normalized to 18S rRNA signals and relative expression is given as fold increase compared to non-vaccinated mice on day 0 p.i. All values are means and half S.E.M. (B) Analysis of TCPOBOP effects on expression of SULT2a1 and CYP7a1. Vaccinated (+ vacc) or non-vaccinated (- vacc) mice were subjected to injection of TCPOBOP (grey columns) or vehicle (black columns) before analysis of SULT2a1 and CYP7a1 expression on the indicated days of P. chabaudi infection using real-time RT-PCR as in (A). (C) TCPOBOP effects on expression of SULT2a were confirmed by Northern blotting. Blot is a representative of three independent experiments. Equal loading of the blot was confirmed by rehybridization with a probe specific for 18S rRNA.