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Table 1 Sequence of primers used to amplify the timeless gene fragments

From: Assessing the molecular divergence between Anopheles (Kerteszia) cruzii populations from Brazil using the timeless gene: further evidence of a species complex

Primers Name

Sequence of primers at 5' → 3'

5'timdeg03

AARGARTTYACNGTNGAYTT (forward)

3'timdeg03

GTNACNARCCARAARAARTG (reverse)

3'cruziitim03

GACGTATCGATCTGCACTT (reverse)

5'cruziitim02

CGCTTCAATGCCGCAAATA (forward)

5'acbatim02a

GCCGCAAATAAGCACCG (forward)

  1. Degenerate and specific primers used to amplify the timeless gene fragments in all Anopheles cruzii populations.