Assessing the molecular divergence between Anopheles (Kerteszia) cruzii populations from Brazil using the timeless gene: further evidence of a species complex

Table 2 Polymorphisms of all An. cruzii populations

Population	n	S	θ	π	D _T	D _FL	F _FL
Florianópolis	24	57 (17)	0.04258 (0.02322)	0.03018 (0.01483)	-1.00660 (-1.24295)	-0.62541 (-1.24456)	-0.87450 (-1.45349)
Cananéia	24	46 (12)	0.03334 (0.01665)	0.02677 (0.01021)	-0.64691 (-1.30282)	-0.47115 (-0.96989)	-0.61709 (-1.24756)
Juquitiba	22	52 (20)	0.03522 (0.02652)	0.03086 (0.02217)	-0.48955 (-0.51723)	-0.47485 (-0.34701)	-0.56076 (-0.46415)
Itatiaia	24	26 (12)	0.01864 (0.01665)	0.01829 (0.01825)	-0.00645 (0.40503)	-0.32168 (0.25917)	-0.25815 (0.35329)
Santa Teresa	12	35 (15)	0.03042 (0.02558)	0.02518 (0.02248)	-0.65598 (-0.41589)	-0.86793 (-0.58337)	-0.92570 (-0.61405)
Bahia	28	24 (9)	0.01661 (0.01099)	0.01035 (0.00571)	-1.31797 (-1.49603)	-0.83982 (-0.91433)	-1.16519 (-1.27249)

n, number of DNA sequences of each population; S, number of polymorphic (segregating) sites; θ, nucleotide diversity based on the total number of mutations (Eta); π, nucleotide diversity based on the average number of pair-wise differences; D_T, Tajima's [40]D; D_FL, Fu & Li's [41]D and F_FL, Fu & Li's [41]F, based on the total number of mutations. In no cases were Tajima's D-values or Fu & Li's D and F-values significant (P > 0.10 in all cases). The values in parentheses were calculated using only coding regions of the timeless gene fragment.

ISSN: 1475-2875