Malaria Journal

Table 2 Ag-ace-1^Dfrequency in West Africa.

From: Ace-1 duplication in Anopheles gambiae: a challenge for malaria control

			M form					S form
#	Locality	N	R	S	D	P -value	N	R	S	D	P -value
1	Abomey	3	-	1	-	-	68	0	0.87	0.13	0.144 ^NS
2	Bohicon	2	-	1	-	-	3	0	0.82	0.18	0.654 ^NS
3	Houegbo	9	-	1	-	-	62	0	0.97	0.03	0.715 ^NS
4	Niaouli	50	-	1	-	-	12	0	0.96	0.04	0.835 ^NS
5	Paouignan	0	-	-	-	-	41	0	0.9	0.1	0.352 ^NS
6	Zogbodomey	13	-	1	-	-	9	0	0.94	0.06	0.808 ^NS
7	Cana	38	-	1	-	-	26	0	0.83	0.17	0.227 ^NS
8	Bembereke	0	-	-	-	-	62	0	0.96	0.04	0.647 ^NS
9	Parakou	0	-	-	-	-	20	0	0.97	0.03	0.873 ^NS
10	Bassila	0	-	-	-	-	76	0	0.97	0.03	0.68 ^NS
11	Tanguieta	0	-	-	-	-	47	0	0.96	0.04	0.673 ^NS
12	Natitingou	0	-	-	-	-	48	0	0.99	0.01	0.918 ^NS
13	Djougou	0	-	-	-	-	46	0	0.97	0.03	0.750 ^NS
14	Dassa	0	-	-	-	-	64	0	0.96	0.04	0.652 ^NS
15	Savalou	0	-	-	-	-	29	0	0.96	0.04	0.789 ^NS
	Total Bénin	115	-	1	-	-	221	0	0.91	0.09	0.052 ^NS
16	Darsalamy	7	-	1	-	-	2	0	0	1	0.096 ^NS
17	Dioulassoba	1	-	1	-	-	23	0.21	0.55	0.24	0.044 *
18	Kuinima	0	-	-	-	-	27	0	0.58	0.42	0.002 **
19	Mombamba	8	-	1	-	-	7	0	0.85	0.15	0.563 ^NS
20	Sabou	2	-	1	-	-	14	0	0.76	0.24	0.198 ^NS
21	Samandeni	20	-	1	-	-	25	0	0.57	0.43	0.002 **
22	Séguéré	10	-	1	-	-	8	0	0.5	0.5	0.049 *
23	Soumousso	32	-	1	-	-	12	0	0.71	0.29	0.153 ^NS
24	Vallée du Kou	86	0	0.96	0.04	0.641 ^NS	80	0.16	0.34	0.51	0.000 ***
25	Yegueresso	8	0	0.87	0.13	0.592 ^NS	2	0	0.71	0.29	0.560 ^NS
26	Boromo	38	0.05	0.95	0	1.000 ^NS	2	0	0.71	0.29	0.560 ^NS
	Total Burkina Faso	212	0.03	0.97	0	1.000 ^NS	202	0.12	0.53	0.35	0.000 ***
27	Toumodi	18	0	0.41	0.59	0.001 ***	0	-	-	-	-
28	Niamoue	24	0.35	0	0.65	0.000 ***	0	-	-	-	-
29	Toumbokro	19	0.23	0.61	0.16	0.195 ^NS	5	0	0	1	0.008 **
30	Yaokoffikro	0	-	-	-	-	19	0.32	0.32	0.35	0.009 **
	Total Ivory Coast	61	0.26	0.4	0.34	0.000 ***	24	0.29	0.29	0.42	0.001 ***
31	Lomé (Togo)	73	0	0.93	0.07	0.391 ^NS	13	0	0.88	0.12	0.531 ^NS

For each population sampled, the number of mosquitoes of each of the S and M molecular forms of An. gambiae is given, together with the estimated frequency of the various alleles: R, S and D for ace-1^R, ace-1^Sand ace-1^D, respectively. A global estimation is also presented for each country sampled. The populations in italics are those in which Ag-ace-1^Dhas been identified by molecular analysis [5]. Finally, the p-value for the test for the presence of ace-1^Dis also given for each population (see Methods), with ^NS for P ≥ 0.05, * for P < 0.05, ** for P < 0.01 and *** for P < 0.001 (except when no estimate was possible, i.e. when all the mosquitoes of a sample were susceptible). The P-values that remained significant after Bonferroni correction (see methods) are presented in bold; P-values no longer significant after Bonferroni correction are shown in italics.

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