Table 1 Oligonucleotide primers for msp1 and msp2 genotyping and fluorescent modifications for the CE method

Segment-specific primers for block 2 of msp1 and block 3 of msp2

In the primary reaction

msp1 F

5'-CTAGAAGCTTTAGAAGATGCAGTATTG-3'

msp1 R

5'-CTTAAATAGTATTCTAATTCAAGTGGATCA -3'

msp2 F

5'-ATGAAGGTAATTAAAACATT GTCTATTATA-3'

msp2 R

5'- CTTTGTTACCATCGGTACATTCTT-3'

Allelic type-specific primers for msp1 in the nested reaction

K1 F

5'- AAATGAAGAAGAAATTACTACAAAAGGTGC-3'

7 bp-tail

K1 R

5'- GCTTGCATCAGCTGGAGGGCTTGCACCAGA-3'

NED™ (yellow)

MAD 20 F

5'- AAATGAAGGAACAAGTGGAACAGCTGTTAC -3'

7 bp-tail

MAD 20 R

5'- ATCTGAAGGATTTGTACGTCTTGAATTACC'-3'

PET^® (red)

RO33 F

5'- TAAAGGATGGAGCAAATACTCAAGTTGTTG-3'

7 bp-tail

RO33 R

5'- CATCTGAAGGATTTGCAGCACCTGGAGATC-3'

VIC^® (green)

Allelic type-specific primers for msp2 in the nested reaction

FC27 F

5'- AATACTAAGAGTGTAGGTGCARATGCTCCA-3'

7 bp-tail

FC27 R

5'- TTTTAT TTG GTGCAT TGCCAGAAC TTG AAC-3'

6-FAM™ (blue)

IC ^c F

5'- AGAAGTATGGCAGAAAGTAAKCCTYCTACT-3'

7 bp-tail

IC^c R

5'- GATTGTAATTCGGGGGATTCAGTTTGTTCG-3'

VIC^® (green)