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Table 2 Genotyping of msp1 and msp2 in laboratory lines in different dilutions and mixtures using CE and gel electrophoresis

From: Optimization and validation of multi-coloured capillary electrophoresis for genotyping of Plasmodium falciparum merozoite surface proteins (msp1 and 2)

   msp1 msp2
   K1 MAD20 RO33 FC27 IC
Lines Densitya CEb gelc CEb gelc CEb gelc CEb gelc CEb gelc
F32 1000 0 0 192.99 205.50 0 0 0 0 524.09 539.10
  100 0 0 193.01 207.06 0 0 0 0 524.05 539.12
  50 0 0 192.94 203.59 0 0 0 0 524.07 539.28
  10 0 0 193.02 201.20 0 0 0 0 524.25 519.80
  5 0 0 192.99 205.10 0 0 0 0 524.06 540.91
  1 0 0 0 0 0 0 0 0 0 0
K1 1000 173.48 177.61 0 0 0 0 372.82 392.41 0 0
  100 173.43 180.28 0 0 0 0 372.85 378.61 0 0
  50 173.30 179.85 0 0 0 0 372.87 381.90 0 0
  10 173.64 182.89 0 0 0 0 372.86 382.01 0 0
  5 173.56 182.51 0 0 0 0 372.85 395.98 0 0
  1 0 0 0 0 0 0 0 0 0 0
7G8 1000 0 0 0 0 157.06 165.55 0 0 493.14 488.25
  100 0 0 0 0 157.35 162.95 0 0 493.13 488.21
  50 0 0 0 0 157.23 167.94 0 0 493.16 472.87
  10 0 0 0 0 157.33 160.40 0 0 493.19 515.93
  5 0 0 0 0 157.19 165.92 0 0 0 508.05
  1 0 0 0 0 0 0 0 0 0 0
F32/K1 1000/10 173.60 177.06 192.98 197.04 0 0 0 0 523.52 509.46
  500/500 173.66 174.49 192.96 194.87 0 0 372.99 380.85 524.25 521.31
  10/1000 173.69 182.86 193.02 192.74 0 0 372.96 379.48 524.19 519.79
K1/7G8 1000/10 173.64 178.01 0 0 157.31 152.59 372.97 364.85 493.51 483.32
  500/500 173.60 175.39 0 0 157.34 161.56 372.98 363.65 493.43 485.01
  10/1000 173.61 172.85 0 0 157.26 161.57 0 0 493.38 500.08
F32/7G8 1000/10 0 0 193.08 190.99 157.49 0 0 0 524.20/492.68 521.99/0
  500/500 0 0 193.08 188.05 157.49 165.59 0 0 524.19/493.47 539.46/0
  10/1000 0 0 193.07 183.33 157.52 163.02 0 0 524.08/493.41 0/493.20
  1. a parasites/μl
  2. b Fragment siz (bp) including 7 bp tail + A, determined by GeneMapper® soft ware after simplex PCR
  3. c Fragment sizes (bp) determined by Quality One 4.4.1 analysis software