Agreement of Plasmodium spp . detection by the three molecular methods. The cytochrome b-based detection method (CYTB) showed the highest number of Plasmodium infections (285 samples), while 271 and 256 infections were observed with the Dot-blot detection (dot18S) and the "standard" nested PCR (Std), respectively. The three methods agreed on the detection of 239 positive and 40 negative samples (agreement rate of 82.8%). Kappa coefficient (Kappa) is calculated between those three methods with 95% Confidence Interval (95% CI).