Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers

Malaria Journal

Table 1 Primers and probes used for Dot-blot 18S rDNA-based detection and primers for cytochrome b-based detection

	5' – 3' sequences	specificity
Dot-blot *18S rDNA* -based detection
Primary PCR
Ps	CTT TCT TgA TTT CTT ggA	P. spp.
PPas	ATT CCT CgT TCA AgA TTA A	P. spp.
Nested PCR
Ns1	CAT ggC CgT TTT Tag TTC gTg AAT AT	P. spp.
Nas new	CAC gCg TgC AgC CTA gTT	P. spp.
Probes
PLAS1	ATA ACg AAC gAg ATC TTA ACC	P. spp.
FAL	CTC TAT TTC TCT CTT CTT TTA AgA	Pf type A, S
VIVA1	AAT ATT ggg ATA CgT AAC AgT	Pv type A
VIVS1	gTT TCT TAA TCg AAT AgC TgA	Pv type S
MAL2b	AgA ATA TAg ATA AAT TgT gCT AA	Pm
OVA3	TgA AAT TgA ATA TAg CTg AAT T	Po
*Cytochrome b* -based detection
Primary PCR
GCDW2	Cgg TCg CgT CCg gTA gCg TCT AAT gCC TAg ACg TAT TCC TgA TTA TCC Ag	P. spp.
GCDW4	CgC ATC ACC TCT ggg CCg CgT gTT TgC TTg ggA gCT gTA ATC ATA ATg Tg	P. spp.
Nested PCR
PLAS1	gAg AAT TAT ggA gTg gAT ggT g	P. spp.
PLAS2	Tgg TAA TTg ACA TCC AAT CC	P. spp.

Specificity of primers and probes is indicated as follows: P. spp. = Plasmodium spp., Pf/Pv/Pm/Po = P. falciparum, P. vivax, P. malariae and P. ovale, respectively, type A/S = stage-specific 18S rDNA type A and S, respectively.

ISSN: 1475-2875