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Table 1 Primers and probes used for Dot-blot 18S rDNA-based detection and primers for cytochrome b-based detection

From: Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers

  5' – 3' sequences specificity
Dot-blot 18S rDNA -based detection
Primary PCR   
Ps CTT TCT TgA TTT CTT ggA P. spp.
PPas ATT CCT CgT TCA AgA TTA A P. spp.
Nested PCR   
Ns1 CAT ggC CgT TTT Tag TTC gTg AAT AT P. spp.
Nas new CAC gCg TgC AgC CTA gTT P. spp.
Probes   
PLAS1 ATA ACg AAC gAg ATC TTA ACC P. spp.
FAL CTC TAT TTC TCT CTT CTT TTA AgA Pf type A, S
VIVA1 AAT ATT ggg ATA CgT AAC AgT Pv type A
VIVS1 gTT TCT TAA TCg AAT AgC TgA Pv type S
MAL2b AgA ATA TAg ATA AAT TgT gCT AA Pm
OVA3 TgA AAT TgA ATA TAg CTg AAT T Po
Cytochrome b -based detection
Primary PCR   
GCDW2 Cgg TCg CgT CCg gTA gCg TCT AAT gCC TAg ACg TAT TCC TgA TTA TCC Ag P. spp.
GCDW4 CgC ATC ACC TCT ggg CCg CgT gTT TgC TTg ggA gCT gTA ATC ATA ATg Tg P. spp.
Nested PCR   
PLAS1 gAg AAT TAT ggA gTg gAT ggT g P. spp.
PLAS2 Tgg TAA TTg ACA TCC AAT CC P. spp.
  1. Specificity of primers and probes is indicated as follows: P. spp. = Plasmodium spp., Pf/Pv/Pm/Po = P. falciparum, P. vivax, P. malariae and P. ovale, respectively, type A/S = stage-specific 18S rDNA type A and S, respectively.