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Table 2 Comparison of malaria diagnosis by microscopy and "standard" nested PCR The comparison between microscopy and "standard" PCR diagnosis is shown for samples collected in the Ping village (n = 102, Table 2A) and for samples collected in the Smach and Pahoy villages (n = 235, Table 2B)

From: Towards high-throughput molecular detection of Plasmodium: new approaches and molecular markers

  Standard PCR (%)
Microscopy Neg Pf Pv Pf+Pv Pf+Pm Pf+Po Pf+Pv+Po Pf+Pv+Pm Pf+Pv+Pm+Po Total   
Neg 20 13   7 1      41 neg (40.2)
Pf 4 20 1 10 6 1   6 1 49 si (50.0)
Pv    1 1       2   
Pf+Pv 1   2 4    1 1 1 10 mi (9.8)
Total 25 33 4 22 7 1 1 7 2 102   
  neg si     mi           
(%) (24.5) (36.3)     (39.2)           
  Standard PCR (%)
Microscopy neg Pf Pv Pm Po Pf+Pv Pf+Pm Pf+Po Pv+Pm Pv+Po Pf+Pv+Po Pf+Pv+Pm Pf+Pv+Pm+Po Total   
Neg 61 47 10 3 2 14 4 2 2 1 1 7 2 156 neg (66.4)
Pf 7 26 5    15 4 1 1   4 6 3 72 si (31.5)
Pv   1 1            2   
Pf+Pv       1      1 2 1 5 mi (2.1)
Total 68 74 16 3 2 30 8 3 3 1 6 15 6 235   
  neg si mi    
(%) (28.9) (40.4) (30.6)    
  1. Pf/Pv/Pm/Po = P. falciparum, P. vivax, P. malariae and P. ovale; neg = negative; si = single infection; mi = mixed infection