Mouse experiments with malarial infection: experiment, parasitaemia and 1H NMR based PCA of urine. A. Each experiment consisted of 3 males (m) and 3 females (f) BALB/c mice of the same litter, housed in individual metabolic cages. The mice were daily sampled for urine for 20 days (13 days post-infection). The arrow indicates the day of infection with Plasmodium berghei ANKA. B. The average parasitaemia profile of the mice as a function of disease progression. C. A representative scores plot showing the trajectory of a single infected animal. Each point corresponds to the day indicated. Black circle = pre-infection, Red triangle = post-infection. The ellipse is a 95% Hotelling's T2 ellipse. R2X (cum) = 0.794 (male), 0.829 (female).