Representative OPLS-DA scores of 1H NMR spectra of mouse urine showing post-infection temporal changes. A-C male. D-F female. Data-points of days -2 to 0 (pre-infection) for 3 mice of a litter have been compared with A, D: days 1 to 4 (early stage infection); B, E: days 5 to 8 (intermediate stage infection) and C, F: days 9 to ~13 (late stage infection). Each point represents a sample corresponding to the day indicated. The first number in the label indicates the mouse. 1 = inf1, 2 = inf2, c = control. Black circle = uninfected, Red triangle = infected. The ellipse is a 95% Hotelling's T2 ellipse. For A and B non-significant orthogonal components had to be calculated. R2X(cum), Q2(cum) = A. 0.569, -0.126; B. 0.531, -0.371; C. 0.925, 0.926; D. 0.752, 0.64; E. 0.558, 0.57; F. 0.911, 0.837.