Table 1 Sequences of the primers used to amplify the Pvmsp-3α and Pvmsp-3β genes of Plasmodium vivax and the Pfmsp-1 and Pfmsp-2 genes of Plasmodium falciparum isolates from Bannu district, Pakistan.

F: 5'-CAGCAGACACCATTTAAGG-3'

95°C 3 min/[94°C 30 s, 54°C 30 s, 68°C 2.5 min] × 35 cycles, 68°C 5 min

[19]

R: 5'-CCGTTTGTTGATTAGTTGC-3'

F: 5'-GACCAGTGTGATACCATTAACC-3'

95°C 3 min/[94°C 30 s, 55°C 30 s, 68°C 2.5 min] × 30 cycles, 68°C 5 min

[19]

R: 5'-ATACTGGTTCTTCGTCTTCAGG-3'

F: 5'-GTATTCTTCGCAACACTC-3'

95°C 3 min/[94°C 30 s, 54°C 30 s, 68°C 2.5 min] × 35 cycles, 68°C 5 min

[20]

R: 5'-CTTCTGATGTTATTTCCAG-3'

F: 5'-CGAGGGGCGAAATTGTAAACC-3'

95°C 3 min/[94°C 30 s, 55°C 30 s, 68°C 2.5 min] × 30 cycles, 68°C 5 min

[20]

R: 5'-GCTGCTTCTTTTGCAAAGG-3'

F: 5'-CTAGAAGCTTTAGAAGATGCAGTATTG-3'

95°C 5 min/[94°C 30 s, 55°C 30 s, 72°C 1 min] × 35 cycles, 72°C 5 min

[17]

R: 5'-CTTAAATAGTATTCTAATTCAAGTGGATCA-3'

F: 5'-AAATGAAGAAGAAATTACTACAAAAGGTGC-3'

95°C 5 min/[94°C 30 s, 56°C 30 s, 72°C 45 s] × 30 cycles, 72°C 5 min

[17]

R: 5'-GCTTGCATCAGCTGGAGGGCTTGCACCAGA-3'

F: 5 '-AAATGAAGGAACAAGTGGAACAGCTGTTAC-3 '

95°C 5 min/[94°C 30 s, 56°C 30 s, 72°C 45 s] × 30 cycles, 72°C 5 min

[17]

R: 5 '-ATCTGAAGGATTTGTACGTCTTGAATTACC-3 '

F: 5 '-TAAAGGATGGAGCAAATACTCAAGTTGTTG-3 '

95°C 5 min/[94°C 30 s, 56°C 30 s, 72°C 45 s] × 30 cycles, 72°C 5 min

[17]

R: 5 '-CATCTGAAGGATTTGCAGCACCTGGAGATC-3 '

F: 5 '-ATGAAGGTAATTAAAACATTGTCTATTATA-3 '

95°C 5 min/[94°C 30 s, 56°C 30 s, 72°C 45 s] × 30 cycles, 72°C 5 min

[17]

R: 5'-CTTTGTTACCATCGGTACATTCTT-3'

F: 5'-AATACTAAGAGTGTAGGTGCARATGCTCCA-3'

95°C 5 min/[94°C 30 s, 56°C 30 s, 72°C 45 s] × 30 cycles, 72°C 5 min

[17]

R: 5 '-TTTTATTTGGTGCATTGCCAGAACTTGAAC-3 '

F: 5 '-AGAAGTATGGCAGAAAGTAAKCCTYCTACT-3 '

95°C 5 min/[94°C 30 s, 56°C 30 s, 72°C 45 s] × 30 cycles, 72°C 5 min

[17]

R: 5 '-GATTGTAATTCGGGGGATTCAGTTTGTTCG-3 '