Figure 4

Effect of sodium aurothiomalate treatment on the parasitemia and survival of Plasmodium berghei -infected mice. A: Original histograms of parasitemia-dependent Syto16 fluorescence in untreated animals (upper panels) and in animals treated daily from day 8 daily with 10 mg/kg b.w. sodium aurothiomalate s.c. (lower panels) 10 (left panels) and 20 (right panels) days after infection with P. berghei. B: Arithmetic means ± SEM of the parasitemia in mice without treatment (open circles, n = 8 mice) or with daily 10 mg/kg b.w. s.c. of sodium aurothiomalate (closed circles, n = 6 mice) as a function of days after infection with P. berghei. Significant difference (* p < 0.05, ** p < 0.01, *** p < 0.001; t-test) from the untreated animals on days 12 - 20. The results presented are one of three independent series. C: Survival of mice without treatment (open circles) or with daily 10 mg/kg b.w. sodium aurothiomalate s.c. (closed circles) as a function of days after infection with Plasmodium berghei. D-E: Arithmetic means ± SEM of the parasitemia in mice without treatment (open circles, n = 4 mice) or with daily 10 mg/kg b.w. s.c. of sodium aurothiomalate (closed circles, n = 4 mice) as a function of days after infection with P. berghei. The parasitemia was determined daily either by staining with Syto16 and subsequent FACS analysis as in B (D) or by daily Giemsa staining of blood smears and light microscopy-dependent analysis (E).