Gal4 constructs for transporting GFP in P. falciparum. The pGal4-GFP construct has the first 147 residues of Gal4p fused to GFP. Expression of Gal4-GFP was controlled by the 5" and 3" flanking sequences of Pfhsp86 and Pbdhfr-ts, respectively. The selection marker was human dihydrofolate reductase (hdhfr), which allows selection by WR99210. A pGal4-GFP construct was divided further into pGal4(1-74)-GFP and pGal4(75-147)-GFP to narrow down the nuclear localization sequence. Rep20 was used to promote plasmid maintenance .