Figure 1

Transcript abundances of rif genes. (A) Transcript abundances of rif genes upon differentiation from asexual to sexual reproduction in a 3D7 parasite line selected with IgG from semi-immune children, 3D7-Dodowa1, an unselected culture of clone 3D7 (RNA from year 2006 and 2008), and an unselected NF54 parasite line. (B) Transcript abundances of rif genes in NF54 sporozoites. Transcript abundances were measured by quantitative PCR using a set of primers that amplify 154 rif genes from clone 3D7/NF54. Transcript abundance of each rif gene is shown as a proportion of the total transcript abundance of rif genes in a sample. Numbered sectors of the pie charts correspond to the following genes: 1, PF13_0006; 2, PFI0025c; 3, PFA0030c; 4, PFL0025c; 5, PFD1230c. Average transcript abundances are shown as the total transcript abundance of rif genes normalized to the average transcript abundance of endogenous control genes, seryl-tRNA synthetase and fructose-bisphosphate aldolase.