Green fluorescent protein (GFP) transfection plasmid. Plasmid pHGBrHrBl-1/2 was constructed and used to transfect P. falciparum blood-stage parasites in vitro. Letters and numbers forming the name of the plasmid are in bold. The plasmid has the H sp86 5' promoter driving G FP expression with a P. berghei 3' terminator region (B), and r ep20 targeting sequence. The drug cassette contains the PfHr pIII 5' promoter driving blasticidin-S-deaminase (BSD) (Bl) with a PfHrpII 3' terminator. Fluorescence and drug cassettes are orientated in a head to tail orientation (5' to 3' end followed by 5' to 3' end) (1/2).