Figure 1

Representative example of the flowcytometric analysis of the IL-10 secreting CD4⁺CD25⁺ population of PBMCs stimulated with malaria extract for 7 days. A. Leukocytes were identified by forward (FSC) and sideward (SSC) scatter. B. Of these leukocytes staining was performed with CD4-PE-Cy7-A and CD25-APC-Cy7-A labeled monoclonal antibodies from BD Pharmingen. C. The CD4⁺CD25⁺ double positive cells were stained intracellularly with IL-4 APC-A and IL-10 PE-A labeled monoclonal antibodies from BD Pharmingen. Panel 1 contains 14% IL-4 single positive CD4⁺CD25⁺ cells, panel 3 contains 2.5% IL-10 single positive CD4⁺CD25⁺ cells, and panel 2 contains 7.2% IL-4⁺IL-10⁺ double positive CD4⁺CD25⁺ cells.