Skip to main content

Table 1 Plasmion enrichment of trophozoites and schizonts in P. falciparum cultures

From: New synchronization method for Plasmodium falciparum

Parasite Parasitaemia before Plasmion (%) Parasitaemia after Plasmion (%) Enrichment factor
  Total R T S Total R T S Total R T S
3D7 11.3 6.1 2.4 2.8 47.2 2.8 26.1 18.3 4.2 0.5 11.1 6.5
3D7 12.1 6.9 3.1 2.1 51.2 2.5 35.9 12.8 4.2 0.4 11.6 6.0
HB3 9.8 4.8 2.6 2.4 62.6 2.1 26.3 34.2 6.4 0.4 10.1 14.3
HB3 10.0 5.0 1.0 4.0 70.0 1.4 16.0 52.6 7.0 0.3 16.0 13.1
XP5 13.0 6.0 3.0 4.0 66.8 2.8 29.0 35.0 5.1 0.5 9.7 8.8
X12 10.2 4.0 1.1 5.1 78.1 2.1 22.0 53.9 7.7 0.5 22.0 10.8
Mean enrichment 5.78 0.4 13.4 9.9
  1. Asexual culture parasitaemia was estimated by examination of 2,000 erythrocytes for each culture, either immediately before or following enrichment with Plasmion. The numbers of ring (R), trophozoite (T) and schizont (S) stages present in each smear were also recorded. Trophozoites were distinguished from ring stage parasites by the appearance of pigment. Schizonts were distinguished from trophozoites by the presence of two or more nuclei. The enrichment factor was calculated by dividing the post-Plasmion treatment parasitaemia by that at pre-treatment.