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Table 1 Plasmion enrichment of trophozoites and schizonts in P. falciparum cultures

From: New synchronization method for Plasmodium falciparum

Parasite

Parasitaemia before Plasmion (%)

Parasitaemia after Plasmion (%)

Enrichment factor

 

Total

R

T

S

Total

R

T

S

Total

R

T

S

3D7

11.3

6.1

2.4

2.8

47.2

2.8

26.1

18.3

4.2

0.5

11.1

6.5

3D7

12.1

6.9

3.1

2.1

51.2

2.5

35.9

12.8

4.2

0.4

11.6

6.0

HB3

9.8

4.8

2.6

2.4

62.6

2.1

26.3

34.2

6.4

0.4

10.1

14.3

HB3

10.0

5.0

1.0

4.0

70.0

1.4

16.0

52.6

7.0

0.3

16.0

13.1

XP5

13.0

6.0

3.0

4.0

66.8

2.8

29.0

35.0

5.1

0.5

9.7

8.8

X12

10.2

4.0

1.1

5.1

78.1

2.1

22.0

53.9

7.7

0.5

22.0

10.8

Mean enrichment

5.78

0.4

13.4

9.9

  1. Asexual culture parasitaemia was estimated by examination of 2,000 erythrocytes for each culture, either immediately before or following enrichment with Plasmion. The numbers of ring (R), trophozoite (T) and schizont (S) stages present in each smear were also recorded. Trophozoites were distinguished from ring stage parasites by the appearance of pigment. Schizonts were distinguished from trophozoites by the presence of two or more nuclei. The enrichment factor was calculated by dividing the post-Plasmion treatment parasitaemia by that at pre-treatment.