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Figure 3 | Malaria Journal

Figure 3

From: Plasmodium falciparum PfA-M1 aminopeptidase is trafficked via the parasitophorous vacuole and marginally delivered to the food vacuole

Figure 3

BFA blocks the p120 form of PfA-M1 in a compartment close to the nucleus. Synchronized parasites were treated for 19 hours in presence of 5 μM BFA (B) or in presence (E) or absence (O) of ethanol, in control cultures. A. Parasites were harvested, isolated by using 0.1% saponin and soluble fractions were blotted with anti-MAP1 antibodies to detect PfA-M1. The p120 form of PfA-M1 is present in BFA-treated cultures while p120, p96 and p68 forms are present in both control cultures, E and O. B. BFA-treated (a) and control parasites in presence of ethanol (c) were analysed by immunofluorescence as previously described [4, 6] by using anti-MAP1 antibodies [6] to detect PfA-M1 (red) and a mouse anti-exp2 [19] serum as positive control (green). Nuclei, stained by using Hoechst 33342 appear in blue. Corresponding phase contrasts are in (b, d). Scale bar, 5 μm.

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