Figure 5

Increasing the concentration of saponin frees p96 but not p68 from pellet to supernatant. Infected erythrocytes at the trophozoite stage were treated with increasing concentrations of saponin from 0.04% (lanes 1 and 2), 0.08% (lanes 3 and 4), 0.1% (lanes 5 and 6) to 0.2% (lanes 7 and 8), separated into supernatant (SNT, lanes 1, 3, 5, 7) and pellet (P, lanes 2, 4, 6, 8) fractions and immunodetected with anti-MAP1 antibodies [6](A, B). Antibodies against PfBip [21] were used as controls (C). The amount of p68 (B) and PfBip (C) in the pellet fraction (parasite) remains constant with increasing concentrations of saponin while the amount of p96 released into the supernatant fraction increases with the saponin concentration (A).