|
fraction
|
p120
|
p96
|
p68
|
PlasI
|
SERP
|
Aldolase
|
|---|
|
A
|
56%
|
58%
|
49%
|
23%
|
15%
|
53%
|
|
B
|
10%
|
9%
|
8%
|
7%
|
25%
|
25%
|
|
C
|
9%
|
7%
|
10%
|
14%
|
29%
|
7%
|
|
D
|
7%
|
8%
|
9%
|
10%
|
9%
|
5%
|
|
E
|
10%
|
10%
|
16%
|
40%
|
17%
|
5%
|
|
F
|
8%
|
8%
|
8%
|
6%
|
5%
|
6%
|
- The p120, p96 and p68 forms of PfA-M1 as compared to Plasmepsin I (PlasI), SERP and Aldolase were quantified in the six fractions of the FV isolation procedure. Fractions are as described on Figure 7A, i.e. fractions A and B corresponding to soluble proteins, fraction C to membranes, vacuoles, debris and fraction E to pure FV. Bands intensities were obtained by scanning each column of the Western Blot on Figure 7B. Values are expressed as a % of the sum of intensities in the six fractions for a given protein.
