fraction | p120 | p96 | p68 | PlasI | SERP | Aldolase |
---|
A
| 56% | 58% | 49% | 23% | 15% | 53% |
B
| 10% | 9% | 8% | 7% | 25% | 25% |
C
| 9% | 7% | 10% | 14% | 29% | 7% |
D
| 7% | 8% | 9% | 10% | 9% | 5% |
E
| 10% | 10% | 16% | 40% | 17% | 5% |
F
| 8% | 8% | 8% | 6% | 5% | 6% |
- The p120, p96 and p68 forms of PfA-M1 as compared to Plasmepsin I (PlasI), SERP and Aldolase were quantified in the six fractions of the FV isolation procedure. Fractions are as described on Figure 7A, i.e. fractions A and B corresponding to soluble proteins, fraction C to membranes, vacuoles, debris and fraction E to pure FV. Bands intensities were obtained by scanning each column of the Western Blot on Figure 7B. Values are expressed as a % of the sum of intensities in the six fractions for a given protein.