Graft of HuRBC and markers of inflammation during the immunomodulation protocol. (A) The percentage of HuRBC grafted in mouse peripheral blood was assessed by FACS by using an anti-human glycophorin A antibody, after several co-injections of HuRBC, clo-lip and NIMP-R14 antibody into the peritoneum (black arrows). (B) Cytokines/chemokines were assessed in mouse sera by FACS using the CBA mouse inflammatory cytokines kit. (C) Leukocyte number. (D) CD43- CD62L+ Ly-6C+ inflammatory monocytes were assessed by FACS. Results are means ± SEM of 2 experiments (n = 15 mice).