Figure 7

Effect of Pg -FET (panel A) and individual compounds (panel B) on NF-kB promoter activity in THP-1 cells stimulated with Hz. Pg-FET, at 50 and 100 μg/ml, inhibited the Hz-induced NF-κB promoter activity by 28% and 50%, respectively. Both individual compounds at 1-10 μM antagonized the Hz-induced promoter activity: -30% and - 41% for EA; -32% and -45% for punicalagin, respectively. Parthenolide (10 μM) was used as reference inhibitor of the NF-κB (~50% inhibition) promoter activity. Data are expressed as mean ± S.D. of triplicate samples. All experiments were reproduced at least three times and, where indicated, representative experiments are shown. Statistical analyses were performed with GraphPad Prism 5 software, using 1-way ANOVA test followed by Bonferroni's post hoc test. The significance was indicated as follows: * p < 0.05; ** p < 0.01.