Figure 8

Effect of Pg -FET (panel A) and individual compounds (panel B) on mkB promoter activity (MMP-9 promoter mutated in the kB site) in THP-1 cells stimulated with Hz. The mutated promoter activity was resistant to Hz induction in comparison to the native promoter (1.3 fold vs 2.5 fold increase) and the inhibitory effect by Pg-FET was less pronounced as well (-20% and -35% at 50 and 100 μg/ml, respectively). Punicalagin at 10 μM inhibited the mutated promoter activity by only 25%, and EA was inactive. Parthenolide (10 μM) was used as reference inhibitor of the NF-κB (~50% inhibition) and MMP-9 (~80% inhibition) promoter activity. Data are expressed as mean ± S.D. of triplicate samples. All experiments were reproduced at least three times. Statistical analyses were performed with GraphPad Prism 5 software, using 1-way ANOVA test followed by Bonferroni's post hoc test. The significance was indicated as follows: * p < 0.05; ** p < 0.01; # p < 0.05 vs ctrl without Hz.