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Table 2 Numbers of false-positive test lines for the different Plasmodium antigens of the RDTs when run with blood of malaria free subjects and substitute liquids

From: Buffer substitution in malaria rapid diagnostic tests causes false-positive results

RDT target antigen

Numbers of RDT brands (n = 27) detecting the target

Total numbers of tests performed with each substitute*

Numbers of false positive test lines when run with substitute liquid

Saline

Distilled water

Tap water

HRP-2

21

98

22

28

36

Pf-pLDH

5

25

8

9

9

Pv-pLDH

9

45

12

21

20

Pan-pLDH

14

70

30

25

33

Aldolase

3

11

1

5

5

  1. *Each RDT brand was assessed with blood of five malaria-free subjects except one HRP-2-based kit and one HRP-2/aldolase-based kit that were assessed with two and one samples respectively.