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Table 2 AMA1 peptides used in ELISpot and ICS assays

From: Identification and localization of minimal MHC-restricted CD8+ T cell epitopes within the Plasmodium falciparum AMA1 protein

Pool

Amino acids

Number peptides

Class II T epitope

Residues

T epitope sequence

Ap1

1-63

13

PL186

14-35

EFTYMIFNGRGQNYWEHPYQKS

   

PL187

41-51

INEHRPKEY

Ap2

53-115

13

PL188

92-103

NLFSSIEIVERS

Ap3

105-167

13

   

Ap4

157-219

13

PL189

188-204

PLMSPMTLDEMRHFYKD

Ap5

209-271

13

PL190

218-229

SRHAGNMIPDND

   

PL191

259-271

NGPRYCNKDE

Ap6

261-323

13

PL192

279-288

AKDISFQNYT

Ap7

313-375

13

PL171

348-366

DQPKQYEQHLTDYEKIKEG

Ap8

365-427

13

PL193

390-402

YKSHGKGYNWGNY

Ap9

417-479

13

PL172

444-461

SLYKNEIMKEIERESKRI

Ap10

469-531

13

   

Ap11

521-583

13

PL194

527-538

EYKDEYADIPEH

   

PL173

571-588

GNAEKYDKMDEPQHYGKS

Ap12

573-622

10

PL173

571-588

GNAEKYDKMDEPQHYGKS

  1. PfAMA1 peptide sequences and residue numbers were based on those of the P. falciparum clone FC27 Gene Bank ID 810891. Previously identified class II AMA1 epitopes (see text) were distributed among peptide pools, except pools 3 and 10. Seven immunodominant pools are indicated in bold type.