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Table 2 AMA1 peptides used in ELISpot and ICS assays

From: Identification and localization of minimal MHC-restricted CD8+ T cell epitopes within the Plasmodium falciparum AMA1 protein

Pool Amino acids Number peptides Class II T epitope Residues T epitope sequence
Ap1 1-63 13 PL186 14-35 EFTYMIFNGRGQNYWEHPYQKS
    PL187 41-51 INEHRPKEY
Ap2 53-115 13 PL188 92-103 NLFSSIEIVERS
Ap3 105-167 13    
Ap4 157-219 13 PL189 188-204 PLMSPMTLDEMRHFYKD
Ap5 209-271 13 PL190 218-229 SRHAGNMIPDND
    PL191 259-271 NGPRYCNKDE
Ap6 261-323 13 PL192 279-288 AKDISFQNYT
Ap7 313-375 13 PL171 348-366 DQPKQYEQHLTDYEKIKEG
Ap8 365-427 13 PL193 390-402 YKSHGKGYNWGNY
Ap9 417-479 13 PL172 444-461 SLYKNEIMKEIERESKRI
Ap10 469-531 13    
Ap11 521-583 13 PL194 527-538 EYKDEYADIPEH
    PL173 571-588 GNAEKYDKMDEPQHYGKS
Ap12 573-622 10 PL173 571-588 GNAEKYDKMDEPQHYGKS
  1. PfAMA1 peptide sequences and residue numbers were based on those of the P. falciparum clone FC27 Gene Bank ID 810891. Previously identified class II AMA1 epitopes (see text) were distributed among peptide pools, except pools 3 and 10. Seven immunodominant pools are indicated in bold type.