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Table 3 ELISpot IFN-γ activity of AMA1 peptide pools and individual 15-mer peptides within these pools

From: Identification and localization of minimal MHC-restricted CD8+ T cell epitopes within the Plasmodium falciparum AMA1 protein

Pool Vol. sfc/m 15-mer peptide Amino acid number Sequence sfc/m Peptide number
Ap1 001 119 Ap1-3 9-23 LLSAFEFTYMINFGR 48 1
Ap1 001 119 Ap1-9 33-47 QNSDVYRPINEHREH 48 2
Ap1 002 161 Ap1-11 41-55 INEHREHPKEYEYPL 180 3
Ap1 005 325 Ap1-11   INEHREHPKEYEYPL 322  
Ap1 002 161 Ap1-12 45-59 REHPKEYEYPLHQEH 66 4
Ap1 005 325 Ap1-12   REHPKEYEYPLHQEH 118  
Ap1 001 119 Ap1-13 49-64 KEYEYPLHQEHTYQQ 42 5
Ap4 005 208 Ap4-5 173-187 NQYLKDGGFAFPTE 50 6
Ap4 002 208 Ap4-9 189-203 LMSPMTLDEMRHFYK 189 7
Ap4 005 310 Ap4-9   LMSPMTLDEMRHFYK 395  
Ap4 002 208 Ap4-10 193-207 MTLDEMRHFYKDNKY 82 8
Ap4 005 208 Ap4-10   MTLDEMRHFYKDNKY 118  
Ap4 005 208 Ap4-11 197-211 EMRHFYKDNKYVKNL 65 9
Ap7 001 131 Ap7-3 310-324 EDIPHVNEFPAIDLF 139 10
Ap7 001 131 Ap7-4 314-328 HVNEFPAIDLFECNK 96 11
Ap7 001 131 Ap7-7 336-350 CNKLVFELSADQPK 77 12
Ap8 012 138 Ap8-6 384-398 FKADRYKSHGKGYNW 143 13
Ap8 001 78 Ap8-11 405-419 ETQKCEIFNVKPCL 48 14
Ap8 005 156 Ap8-11   ETQKCEIFNVKPCL 218  
Ap8 008 78 Ap8-11   ETQKCEIFNVKPCL 48  
Ap8 005 156 Ap8-12 409-423 CEIFNVKPTCLINNS 127 15
Ap10 001 172 Ap10-13 517-531 TSNNEVVVKEEYDE 233 16
Ap10 012 193 Ap10-13   TSNNEVVVKEEYDE 250  
  1. A total of 16 individual peptides were identified from a total of 91 15-mer peptides from seven active pools. Peptides Ap1-11, Ap1-12, Ap4-9, Ap4-10, Ap8-11 and Ap10-13 stimulated strong responses in more than one volunteer.