Identification and localization of minimal MHC-restricted CD8+ T cell epitopes within the Plasmodium falciparum AMA1 protein

Table 3 ELISpot IFN-γ activity of AMA1 peptide pools and individual 15-mer peptides within these pools

Pool	Vol.	sfc/m	15-mer peptide	Amino acid number	Sequence	sfc/m	Peptide number
Ap1	001	119	Ap1-3	9-23	LLSAFEFTYMINFGR	48	1
Ap1	001	119	Ap1-9	33-47	QNSDVYRPINEHREH	48	2
Ap1	002	161	Ap1-11	41-55	INEHREHPKEYEYPL	180	3
Ap1	005	325	Ap1-11		INEHREHPKEYEYPL	322
Ap1	002	161	Ap1-12	45-59	REHPKEYEYPLHQEH	66	4
Ap1	005	325	Ap1-12		REHPKEYEYPLHQEH	118
Ap1	001	119	Ap1-13	49-64	KEYEYPLHQEHTYQQ	42	5
Ap4	005	208	Ap4-5	173-187	NQYLKDGGFAFPTE	50	6
Ap4	002	208	Ap4-9	189-203	LMSPMTLDEMRHFYK	189	7
Ap4	005	310	Ap4-9		LMSPMTLDEMRHFYK	395
Ap4	002	208	Ap4-10	193-207	MTLDEMRHFYKDNKY	82	8
Ap4	005	208	Ap4-10		MTLDEMRHFYKDNKY	118
Ap4	005	208	Ap4-11	197-211	EMRHFYKDNKYVKNL	65	9
Ap7	001	131	Ap7-3	310-324	EDIPHVNEFPAIDLF	139	10
Ap7	001	131	Ap7-4	314-328	HVNEFPAIDLFECNK	96	11
Ap7	001	131	Ap7-7	336-350	CNKLVFELSADQPK	77	12
Ap8	012	138	Ap8-6	384-398	FKADRYKSHGKGYNW	143	13
Ap8	001	78	Ap8-11	405-419	ETQKCEIFNVKPCL	48	14
Ap8	005	156	Ap8-11		ETQKCEIFNVKPCL	218
Ap8	008	78	Ap8-11		ETQKCEIFNVKPCL	48
Ap8	005	156	Ap8-12	409-423	CEIFNVKPTCLINNS	127	15
Ap10	001	172	Ap10-13	517-531	TSNNEVVVKEEYDE	233	16
Ap10	012	193	Ap10-13		TSNNEVVVKEEYDE	250

A total of 16 individual peptides were identified from a total of 91 15-mer peptides from seven active pools. Peptides Ap1-11, Ap1-12, Ap4-9, Ap4-10, Ap8-11 and Ap10-13 stimulated strong responses in more than one volunteer.

ISSN: 1475-2875