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Table 5 Predicted CD8+ T cell-restricted epitopes within AMA1 15-mer peptides specific for each volunteer

From: Identification and localization of minimal MHC-restricted CD8+ T cell epitopes within the Plasmodium falciparum AMA1 protein

Peptide number

Predicted epitope

Amino acid

number

IC50 nM

HLA restriction

HLA supertype

Epitope number

1

LLSAFEFTYMINF GR

13-21

5

B*1801

B44

E1

2

QNSDVYRPINEH REH

35-44

14991

B*4402

B44

E2

3

INEHREHPKEYEYPL

47-55

18

B*0801

B08

E3

4

REHPKEYEYPL HQEH

     

5

KEYEYPLHQEH TYQQ

51-59

39

B*1801

B44

E4

6

NQYLKDGGFAF PTE

175-183

142

B*0801

B08

E5

7

LMSPMTLDEMRHFYK

194-202

17

A*0101

A01

E6

8

MTLDEMRHFYK DNKY

     

9

EMRHFYKDNKY VKNL

198-207

4742

A*0101

A01

E7

10

EDIPHVNEFPAIDLF

327-335

7

B*1801

B44

E8

11

HVNEFPAIDLF ECNK

     

12

CNKLVFELSA DQPK

339-346

5708

A*0201

A02

E9

13

FKADRYKSHGKGY NW

389-397

201

A*3002

A01

E10

14

ETQKCEIFNV KPCL

405-414

142

A*6802

A02

E11

 

ETQKCEIFNV KPCL

406-414

919

A*0201

A02

E12

15

CEIFNVKPTCL INNS

410-419

617

A*6802

A02

E13

16

TSNNEVVVKEEY DE

520-528

15

B*1801

B44

E14

  1. The 15-mer peptides that were recognized by the volunteers (Table 3) were analysed by NetMHC web-based software to predict potential high affinity HLA binding by minimal CD8+ T cell epitopes within each 15-mer. Each minimal epitope was specific for a known HLA allele within each supertype. Those minimal epitopes with the strongest binding affinities for the HLA supertype of each volunteer were selected. The Table shows the minimal epitopes in bold. Two different HLA-restricted epitopes were predicted with peptide 14.