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Table 1 Primer sequences for asexual P. falciparum DNA detection

From: A method of active case detection to target reservoirs of asymptomatic malaria and gametocyte carriers in a rural area in Southern Province, Zambia

Target: CYT-B (will detect all species of human Plasmodium)
Primary (GCD) F 5' CGG TCG CGT CCG GTA GCG TCT AAT GCC TAG ACG TAT TCC TGA TTA TCC AG 3'
  R 5' CGC ATC ACC TCT GGG CCG CGT GTT TGC TTG GGA GCT GTA ATC ATA ATG TG 3'
Nested (PLAS) F 5' GAG AAT TAT GGA GTG GAT GGT G 3'
  R 5' TGG TAA TTG ACA TCC AAT CC 3'
Target: MSP-2 (will detect P. falciparum only)
Primary F 5' ATG AAG GTA ATT AAA ACA TTG TCT ATT ATA '3
(M20) R 5' CTT TGT TAC CAT CGG TAC ATT CTT '3
Nested (IC) F 5' AGA AGT ATG GCA GAA AGT AAK CCT YCT ACT 3'
  R 5' GAT TGT AAT TCG GGG GAT TCA GTT TGT TCG 3'