Table 1 Primer sequences for asexual P. falciparum DNA detection
Target: CYT-B (will detect all species of human Plasmodium) | ||
|---|---|---|
Primary (GCD) | F | 5' CGG TCG CGT CCG GTA GCG TCT AAT GCC TAG ACG TAT TCC TGA TTA TCC AG 3' |
R | 5' CGC ATC ACC TCT GGG CCG CGT GTT TGC TTG GGA GCT GTA ATC ATA ATG TG 3' | |
Nested (PLAS) | F | 5' GAG AAT TAT GGA GTG GAT GGT G 3' |
R | 5' TGG TAA TTG ACA TCC AAT CC 3' | |
Target: MSP-2 (will detect P. falciparum only) | ||
Primary | F | 5' ATG AAG GTA ATT AAA ACA TTG TCT ATT ATA '3 |
(M20) | R | 5' CTT TGT TAC CAT CGG TAC ATT CTT '3 |
Nested (IC) | F | 5' AGA AGT ATG GCA GAA AGT AAK CCT YCT ACT 3' |
R | 5' GAT TGT AAT TCG GGG GAT TCA GTT TGT TCG 3' | |