Table 2 Primer sequences for P. falciparum gametocytes using RT-PCR
Target gene: Pfs25 | ||
|---|---|---|
Primary | 491(F) | 5'-ATC GAT ATG AAT AAA CTT TAC AGT TTG TTT CT-3' |
492 (R) | 5'-GAA TTC TTA CAT TAT AAA AAA GCA TAC TC-3' | |
Nested | 25-1(F) | 5'-TAA TGC GAA AGT TAC CGT GG-3' |
25-2 (R) | 5'-TCC ATC AAC AGC TTT ACA GG-3' | |