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Table 1 Primer and probe sequences

From: Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women

Assay

Reagent

Sequencec

Pfldh

Primersa

P. falciparum LDH forward

ACG ATT TGG CTG GAG CAG AT

  

P. falciparum LDH reverse

TCT CTA TTC CAT TCT TTG TCA CTC TTT C

 

Probeb

TaqMan probe (P. falciparum LDH)

FAM-AGT AAT AGT AAC AGC TGG ATT TAC CAA GGC CCC A-TAMRA

Species-specific

Primersa

P. falciparum 18 S rDNA forward

ATT GCT TTT GAG AGG TTT TGT TAC TTT

  

P. falciparum 18 S rDNA reverse

GCT GTA GTA TTC AAA CAC AAT GAA CTC AA

  

P. ovale 18 S rDNA forward

CCG ACT AGG TTT TGG ATG AAA GAT TTT T

  

P. ovale 18 S rDNA reverse

CAA CCC AAA GAC TTT GAT TTC TCA TAA

  

P. malariae 18 S rDNA forward

AGT TAA GGG AGT GAA GAC GAT CAG A

  

P. malariae 18 S rDNA reverse

CAA CCC AAA GAC TTT GAT TTC TCA TAA

  

Human GAPDH forward

CCT CCC GCT TCG CTC TCT

  

Human GAPDH reverse

GCT GGC GAC GCA AAA GA

 

MGB probesb

P. falciparum

FAM-CAT AAC AGA CGG GTA GTC AT

  

P. ovale

VIC-CGA AAG GAA TTT TCT TAT T

  

P. malariae

FAM-ATG AGT GTT TCT TTT AGA TAG C

  

Human GAPDH

VIC-CCT CCT GTT CGA CAG TCA GCC GC

  1. a Synthesized by MWG/Operon Biotech (High Point, NC) and re-suspended in molecular-grade water. LDH, lactate dehydrogenase. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
  2. b Synthesized by Applied Biosystems (Foster City, CA) and diluted in Tris-EDTA Buffer (FisherBioTech, Fair Lawn, NJ). LDH, lactate dehydrogenase. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
  3. c FAM and VIC denote fluorescent dyes. TAMRA denotes the quencher 6-carboxytetramethylrhodamine. MGB, minor groove binding probes.