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Table 1 Primer and probe sequences

From: Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women

Assay Reagent Sequencec
Pfldh Primersa P. falciparum LDH forward ACG ATT TGG CTG GAG CAG AT
   P. falciparum LDH reverse TCT CTA TTC CAT TCT TTG TCA CTC TTT C
  Probeb TaqMan probe (P. falciparum LDH) FAM-AGT AAT AGT AAC AGC TGG ATT TAC CAA GGC CCC A-TAMRA
Species-specific Primersa P. falciparum 18 S rDNA forward ATT GCT TTT GAG AGG TTT TGT TAC TTT
   P. falciparum 18 S rDNA reverse GCT GTA GTA TTC AAA CAC AAT GAA CTC AA
   P. ovale 18 S rDNA forward CCG ACT AGG TTT TGG ATG AAA GAT TTT T
   P. ovale 18 S rDNA reverse CAA CCC AAA GAC TTT GAT TTC TCA TAA
   P. malariae 18 S rDNA forward AGT TAA GGG AGT GAA GAC GAT CAG A
   P. malariae 18 S rDNA reverse CAA CCC AAA GAC TTT GAT TTC TCA TAA
   Human GAPDH forward CCT CCC GCT TCG CTC TCT
   Human GAPDH reverse GCT GGC GAC GCA AAA GA
  MGB probesb P. falciparum FAM-CAT AAC AGA CGG GTA GTC AT
   P. ovale VIC-CGA AAG GAA TTT TCT TAT T
   P. malariae FAM-ATG AGT GTT TCT TTT AGA TAG C
   Human GAPDH VIC-CCT CCT GTT CGA CAG TCA GCC GC
  1. a Synthesized by MWG/Operon Biotech (High Point, NC) and re-suspended in molecular-grade water. LDH, lactate dehydrogenase. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
  2. b Synthesized by Applied Biosystems (Foster City, CA) and diluted in Tris-EDTA Buffer (FisherBioTech, Fair Lawn, NJ). LDH, lactate dehydrogenase. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
  3. c FAM and VIC denote fluorescent dyes. TAMRA denotes the quencher 6-carboxytetramethylrhodamine. MGB, minor groove binding probes.