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Table 4 Detection of malaria parasites by microscopy, the pfldh real-time PCR assay and the species-specific real-time PCR assay

From: Comparison of real-time PCR and microscopy for malaria parasite detection in Malawian pregnant women

 

Organism(s) detected by each method (No. of samples)

Malaria-causing speciesa

Microscopy, thin and thick blood smears

Real-Time PCR, pfldh assay

Real-Time PCR, species-specific assayb

P. falciparum

P. falciparum (11)

P. falciparum (10)

-

  

Negative (1)

Negative (1)

Non- P. falciparum

P. malariae (3)

P. falciparum (1)

P. falciparum (1)

  

Negative (2)

P. malariae (1)

Negative (1)

Negative

Negative (461)

P. falciparum (40)

P. falciparum (37)

P. falc. + P. malariae (2)

Negative (1)

  

Negative (421)

-

Total

475

475

44

  1. a Malaria species are classified according to the diagnosis obtained from the blood smear examination.
  2. b Species-specific assay is conducted for all specimens with discordant smear and pfldh PCR assay results (44).