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Table 1 Comparison of the frequency of the kdr-e and kdr-w mutations using DNA sequence, and hydrolysis probe analysis with and without adjustment of the allelic discrimination axes.

From: A comparison of DNA sequencing and the hydrolysis probe analysis (TaqMan assay) for knockdown resistance (kdr) mutations in Anopheles gambiae from the Republic of the Congo

  Genotypes
Detection method Ser-Phe Ser-Ser Phe-Phe Ser-Leu Phe-Leu No ID
DNA sequence 29
(55.8%)
7
(13.5%)
14
(26.9%)
1
(1.9%)
1
(1.9%)
0
(0%)
Hydrolysis probe analysis without adjustment 6
(11.5%)
6
(11.5%)
30
(57.8%)
1
(1.9%)
1
(1.9%)
8
(15.4%)
Hydrolysis probe analysis with adjustment 27
(51.9%)
8
(15.4%)
15
(28.9%)
1
(1.9%)
1
(1.9%)
0
(0%)
  1. A total samples were 52 specimens. Ser = L1014 S (kdr-e); Phe = L1014F (kdr-w); Leu = Wild type; No ID = failed to amplify