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Table 1 Comparison of the frequency of the kdr-e and kdr-w mutations using DNA sequence, and hydrolysis probe analysis with and without adjustment of the allelic discrimination axes.

From: A comparison of DNA sequencing and the hydrolysis probe analysis (TaqMan assay) for knockdown resistance (kdr) mutations in Anopheles gambiae from the Republic of the Congo

 

Genotypes

Detection method

Ser-Phe

Ser-Ser

Phe-Phe

Ser-Leu

Phe-Leu

No ID

DNA sequence

29

(55.8%)

7

(13.5%)

14

(26.9%)

1

(1.9%)

1

(1.9%)

0

(0%)

Hydrolysis probe analysis without adjustment

6

(11.5%)

6

(11.5%)

30

(57.8%)

1

(1.9%)

1

(1.9%)

8

(15.4%)

Hydrolysis probe analysis with adjustment

27

(51.9%)

8

(15.4%)

15

(28.9%)

1

(1.9%)

1

(1.9%)

0

(0%)

  1. A total samples were 52 specimens. Ser = L1014 S (kdr-e); Phe = L1014F (kdr-w); Leu = Wild type; No ID = failed to amplify