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Table 1 Comparison of the frequency of the kdr-e and kdr-w mutations using DNA sequence, and hydrolysis probe analysis with and without adjustment of the allelic discrimination axes.

From: A comparison of DNA sequencing and the hydrolysis probe analysis (TaqMan assay) for knockdown resistance (kdr) mutations in Anopheles gambiae from the Republic of the Congo

  Genotypes
Detection method Ser-Phe Ser-Ser Phe-Phe Ser-Leu Phe-Leu No ID
DNA sequence 29 (55.8%) 7 (13.5%) 14 (26.9%) 1 (1.9%) 1 (1.9%) 0 (0%)
Hydrolysis probe analysis without adjustment 6 (11.5%) 6 (11.5%) 30 (57.8%) 1 (1.9%) 1 (1.9%) 8 (15.4%)
Hydrolysis probe analysis with adjustment 27 (51.9%) 8 (15.4%) 15 (28.9%) 1 (1.9%) 1 (1.9%) 0 (0%)
  1. A total samples were 52 specimens. Ser = L1014 S (kdr-e); Phe = L1014F (kdr-w); Leu = Wild type; No ID = failed to amplify