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Figure 4 | Malaria Journal

Figure 4

From: A protein-centric approach for the identification of folate enzymes from the malarial parasite, Plasmodium falciparum, using OFFGEL™ solution-based isoelectric focussing and mass spectrometry

Figure 4

Theoretical and observed pH ranges of individual OFFGEL™ fractions. A. The theoretical pH range was calculated by dividing by 12 the 7 available pH units between pH 3 and 10 to give a value of approximately 0.6 pH units for the range covered by each fraction. The median value for each fraction was then plotted. The observed pH for each fraction was calculated by averaging the pH measured for equivalent fractions from three OFFGEL™ separations. B. Plots of the median theoretical and mean observed pH values for each OFFGEL™ fraction. The standard deviation for each individual OFFGEL™ fraction pH measurement is also displayed.

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