Phylogenetic tree based on the gene encoding the internal transcribed spacer region 2 (ITS2) of Anopheles hyrcanus Group produced by the neighbour-joining method. Those highlighted in bold are sequences obtained from Singapore mosquitoes and clustered in An. sinensis clade. Figures on the branches are bootstrap percentages based on 1000 replicates and only those above 70% are shown. Anopheles barbirostris were used as outgroup. GenBank accession numbers are in brackets.