Comparison of PCT Estimates from daily microscopy versus qPCR. Upper panel depicts daily microscopy data. Initial parasite density was converted into % of pre-treatment level. Patient 3 had very high initial parasitemia (39%). The poor response of patient 4 over the initial 48 hr of QN treatment led to substitution with AP. Lower panel depicts qPCR data, analysed using the ΔΔCT calculation. Relative density of parasite DNA at each sample time point was converted to percentage of starting abundance in order to compare it with microscopy.