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Table 1 Amplification primers and double-labelled hydrolysis probes for PgMET and HumTuBB.

From: Measuring the efficacy of anti-malarial drugs in vivo: quantitative PCR measurement of parasite clearance

Primer/Probe 5' Fluorophore Sequence 3' Quencher
PgMET_F1   5'-TGAAAGCAGCGTAGCTCAGA  
PgMET_R2   5'-CGCGTGGTTTCGATCCACG  
PgMET_pB FAM 5'-GGGGCTCATAACCCCCAGGA BHQ2
HumTuBB_F2   5'-AAGGAGGTCGATGAGCAGAT  
HumTuBB_R2   5'-GCTGTCTTGACATTGTTGGG  
HumTuBB_Joe JOE 5'-TTAACGTGCAGAACAAGAACAGCAGCT BHQ2