Figure 2From: Insect cells are superior to Escherichia coli in producing malaria proteins inducing IgG targeting PfEMP1 on infected erythrocytesPurity of recombinant PFD1235w protein domains expressed in insect cells and E. coli. (A and C) Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of 2 μg/lane and (B and D) Western blotting of 0.2 μg/lane recombinant protein. (A and B) Insect cell produced protein (1-2) NTS-CIDR1α, (3-4) DBL1α-CIDR1α, (5-6) CIDR1α, (7-8) DBL2β, (9-10) DBL3β, (11-12) DBL4γ, (13-14) DBL5δ-CIDR2β, (15-16) DBL5δ, (17-18) CIDR2β and (C and D) E. coli produced (1-2) DBL1α-CIDR1α, (3-4) CIDR1α, (5-6) DBL2β, (7-8) DBL3β, (9-10) DBL4γ, (11-12) DBL5δ-CIDR2β, (13-14) DBL5δ. M: ProSieve Color Protein marker (Lonza). Samples were reduced using DTT (+) or non-reduced (-). Arrows indicate identified protein band.Back to article page