Purity of recombinant PFD1235w protein domains expressed in insect cells and E. coli. (A and C) Sodium dodecyl sulfate-polyacrylamide (SDS-PAGE) gel electrophoresis of 2 μg/lane and (B and D) Western blotting of 0.2 μg/lane recombinant protein. (A and B) Insect cell produced protein (1-2) NTS-CIDR1α, (3-4) DBL1α-CIDR1α, (5-6) CIDR1α, (7-8) DBL2β, (9-10) DBL3β, (11-12) DBL4γ, (13-14) DBL5δ-CIDR2β, (15-16) DBL5δ, (17-18) CIDR2β and (C and D) E. coli produced (1-2) DBL1α-CIDR1α, (3-4) CIDR1α, (5-6) DBL2β, (7-8) DBL3β, (9-10) DBL4γ, (11-12) DBL5δ-CIDR2β, (13-14) DBL5δ. M: ProSieve Color Protein marker (Lonza). Samples were reduced using DTT (+) or non-reduced (-). Arrows indicate identified protein band.