Recognition of native PFD1235w expressed on the surface of 3D7
IE. The surface antibody reactivity of rat antisera raised against (A1 and B1) insect cells and (A2 and B2) E. coli produced DBL1α-CIDR1α and DBL4γ was measured by flow cytometry. (C) The antibody surface reactivity of rat antisera raised against insect cell produced DBL3β. Antisera were pre-incubated with excess homologous immunizing protein, heterologous produced protein, PBS or irrelevant protein as indicated. The percentage of reactivity left following depletion was calculated as described in the Methods section.