Human antibody reactivity to PFD1235w. Percentage antibody responders to recombinant PFD1235w domains produced in baculovirus-infected insect cells (A1-C1) and in E. coli (A2-C2). The antibody reactivity of plasma obtained from individuals living in three Tanzanian villages (A) Magamba, (B) Ubiri, and (C) Mgome of low, moderate, and high malaria transmission intensity was measured by Luminex. Each age group (2-4, 5-9, 10-14, and 15-19 years) included 15 individuals. The percentage responders was categorized into six different intervals: 0-15 (white), 16-30 (lighter gray), 31-45 (light gray), 46-60 (dark grey), 61-75 (darker grey), and >76 (black).