Molecular evidence for the occurrence of a new sibling species within the Anopheles (Kerteszia) cruzii complex in south-east Brazil

Table 3 Genetic differentiation between An. cruzii populations

Populations	F _ST(1)	P-value	F _ST(2)	P-value	D _xy	D _a	S _s	S _f	S ₁	S ₂
1. Florianópolis × Cananéia	0.0845	0.013	0.1445	0.004	0.0220	0.0029	07	00	03	02
2. Cananéia × Santa Teresa	0.1811	0.000	0.1827	0.007	0.0339	0.0061	04	00	05	07
3. Florianópolis × Santa Teresa	0.1575	0.000	0.3196	0.000	0.0307	0.0075	04	00	06	07
4. Cananéia × Juquitiba	0.2673	0.000	0.2008	0.000	0.0216	0.0064	06	00	03	02
5. Santa Teresa × Juquitiba	0.2661	0.000	0.4267	0.000	0.0296	0.0104	04	00	07	04
6. Florianópolis × Juquitiba	0.3616	0.000	0.5238	0.000	0.0231	0.0126	05	00	05	03
7. Cananéia × Itatiaia	0.6105	0.000	0.6627	0.000	0.0597	0.0362	03	04	06	07
8. Santa Teresa × Itatiaia	0.6557	0.000	0.5980	0.000	0.0630	0.0355	02	03	09	08
9. Florianópolis × Itatiaia	0.6812	0.000	0.7355	0.000	0.0564	0.0375	02	04	08	08
10. Juquitiba × Itatiaia	0.7710	0.000	0.8078	0.000	0.0618	0.0470	02	04	06	08

F_ST, pair-wise estimates of population differentiation (see text for more details); P-value, significance of F_STvalues (evaluated by 1,000 random permutations). D_xy, average number of nucleotide substitutions per site between populations [39]; D_a, number of net nucleotide substitutions per site between populations [39]. S₁, number of polymorphic sites exclusive to the first population shown in the first column. S₂, number of polymorphic sites exclusive to the second population shown in the first column. S_s, number of shared polymorphisms between the two populations. S_f, number of fixed differences between the two populations. These values were calculated with P_ROS_EQ v 2.91 [28] using the alignment shown in Additional file 2 and considering the gaps as single mutations.

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