From: A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi
Template | Concentration | Plasprobe Ct* | Pk probe Ct* |
---|---|---|---|
Plasmid (copies/μL) | 1 × 106 | 23.3 | 20.5 |
 | 1 × 105 | 26.7 | 23.6 |
 | 1 × 104 | 30.3 | 27.7 |
 | 1 × 103 | 34.1 | 31.0 |
 | 1 × 102 | 37.6 | 34.4 |
 | 10 | 40.3 | 37.5 |
 | 1 | ND‡ | ND‡ |
gDNA†(parasites/μL blood) | 4480 | 26.7 | 25.6 |
 | 480 | 28.5 | 27.4 |
 | 48 | 32.2 | 31.1 |
 | 4.8 | 35.8 | 34.7 |
 | 0.48 | 40.2 | 38.8 |
 | 0.048 | 42.4 | 39.5 |
 | 0.0048 | ND‡ | ND‡ |