A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi

Malaria Journal

Table 1 Detection of P. knowlesi target gene in plasmid KH33 and genomic DNA

Template	Concentration	Plasprobe Ct*	Pk probe Ct*
Plasmid (copies/μL)	1 × 10⁶	23.3	20.5
	1 × 10⁵	26.7	23.6
	1 × 10⁴	30.3	27.7
	1 × 10³	34.1	31.0
	1 × 10²	37.6	34.4
	10	40.3	37.5
	1	ND‡	ND‡
gDNA†(parasites/μL blood)	4480	26.7	25.6
	480	28.5	27.4
	48	32.2	31.1
	4.8	35.8	34.7
	0.48	40.2	38.8
	0.048	42.4	39.5
	0.0048	ND‡	ND‡

*Cycle threshold.
†Genomic DNA extracted from a clinical sample with single P. knowlesi infection.
‡ND, not detected.

ISSN: 1475-2875