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Table 1 Detection of P. knowlesi target gene in plasmid KH33 and genomic DNA

From: A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi

Template

Concentration

Plasprobe Ct*

Pk probe Ct*

Plasmid (copies/μL)

1 × 106

23.3

20.5

 

1 × 105

26.7

23.6

 

1 × 104

30.3

27.7

 

1 × 103

34.1

31.0

 

1 × 102

37.6

34.4

 

10

40.3

37.5

 

1

ND‡

ND‡

gDNA†(parasites/μL blood)

4480

26.7

25.6

 

480

28.5

27.4

 

48

32.2

31.1

 

4.8

35.8

34.7

 

0.48

40.2

38.8

 

0.048

42.4

39.5

 

0.0048

ND‡

ND‡

  1. *Cycle threshold.
  2. †Genomic DNA extracted from a clinical sample with single P. knowlesi infection.
  3. ‡ND, not detected.