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Table 1 Detection of P. knowlesi target gene in plasmid KH33 and genomic DNA

From: A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi

Template Concentration Plasprobe Ct* Pk probe Ct*
Plasmid (copies/μL) 1 × 106 23.3 20.5
  1 × 105 26.7 23.6
  1 × 104 30.3 27.7
  1 × 103 34.1 31.0
  1 × 102 37.6 34.4
  10 40.3 37.5
  1 ND‡ ND‡
gDNA†(parasites/μL blood) 4480 26.7 25.6
  480 28.5 27.4
  48 32.2 31.1
  4.8 35.8 34.7
  0.48 40.2 38.8
  0.048 42.4 39.5
  0.0048 ND‡ ND‡
  1. *Cycle threshold.
  2. †Genomic DNA extracted from a clinical sample with single P. knowlesi infection.
  3. ‡ND, not detected.