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Table 2 Assay specificity for P. knowlesi

From: A TaqMan real-time PCR assay for the detection and quantitation of Plasmodium knowlesi

Sample Code

Identification by nested PCR assay

Plasprobe Ct*

Pk probe Ct*

MISC23

P. vivax

31.2

ND†

CDK123

P. vivax

30.7

ND†

CDK118

P. vivax

27.6

ND†

SKS358

P. vivax

26.7

ND†

KH116

P. falciparum

25.1

ND†

CDK135

P. falciparum

26.7

ND†

CDK67

P. malariae

37.0

ND†

KH352

P. ovale

26.5

ND†

P. cynomolgi

Plasmodium-positive

15.0

ND†

P. inui

Plasmodium-positive

13.0

ND†

  1. *Cycle threshold.
  2. †ND, not detected.