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Figure 2 | Malaria Journal

Figure 2

From: Automated haematology analysis to diagnose malaria

Figure 2

Cell-Dyn 4000 scatter-plots of samples with no malaria parasites, P. falciparum and P. vivax. A. Haemozoin detection by depolarization in the NEU-EOS scatter-plot: Haemozoin-containing monocytes (purple dots) in eosinophil area (purple arrows). Large black-coded population (black arrow) in P. vivax infection (right scatter-plot). B. Detection by size and depolarized side-scatter in the 'mono poly I' scatter-plot: No difference between 'no malaria' and P. falciparum, while a large population of small size black-coded events appears in the sample with P. vivax. C. Detection of parasite DNA in the nucleated red blood cells (NRBC) scatter-plot by propidium iodide staining: No difference between 'no malaria' and P. falciparum while a large population of black-coded events with high degree of FL3 fluorescence appears in the P. vivax case. Black-coded events may represent parasites (see text), 90 Dgrnlrty: 90° depolarization side-scatter; 90° lobular: 90° side-scatter; 0° Size: forward-scatter; FL3-DNA: fluorescent detection of propidium iodide. *Colour code for events displayed is, blue: lymphocytes; purple: monocytes; orange: neutrophils; green: eosinophils; red: erythrocytes; black: not classified.

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