Automated haematology analysis to diagnose malaria

Campuzano-Zuluaga, Germán; Hänscheid, Thomas; Grobusch, Martin P

doi:10.1186/1475-2875-9-346

Malaria Journal

Table 1 Summary of studies evaluating the malaria diagnostic accuracy of Cell-Dyn series analysers using the side-scatter/depolarized side-scatter plot abnormal depolarizing events criterion. §

From: Automated haematology analysis to diagnose malaria

First author, year and country	Number of participants and diagnoses	Index test criterion^¶	Sensitivity %	Specificity %
Mendelow, 1999, South Africa [18]	Total: 224 directed samples from 175 patients, P. falciparum: 93, Species not specified: 2	CD** 3500 ≥1 depolarizing events‡	72	96
Hänscheid, 2001, Portugal† [39]	Total: 174, P. falciparum: 48, P. vivax: 6, P. ovale: 1, P. malariae: 2	CD 3500 ≥2 depolarizing events	95	88
Wever, 2002, The Netherlands† [36]	Total: 113, P. falciparum: 46, P. vivax: 5, P. ovale: 4, no differentiation for P. vivax or P. ovale: 3	CD 3500 Either ≥1 depolarizing events or pseudoreticulocytosis	62	96
Grobusch, 2003, Germany† [27]	Total: 403, P. falciparum: 87, P. vivax: 13, P. ovale: 5, P. malariae: 2	CD 3000 ≥1 depolarizing events	48.6	96.2
Scott, 2003, South Africa [35]	Total: 831, P. falciparum: 334, P. vivax: 7, P. ovale: 1, P. malariae: 2, mixed or unspecified: 6	CD 4000 ≥1 depolarizing events	80.2	87.3
Suh, 2003, South Korea [32]	Total: 168, P. vivax: 68	CD 4000 ≥1 depolarizing events	91.2	100
Dromigny, 2005, Senegal [30]	Total: 799 (directed: suspected of malaria 123, non-suspected random samples 676) P. falciparum: 68, treated or subclinical: 83	CD 3200 ≥1 depolarizing events	Directed 92.9 Random 90.2	Directed 93.8 Random 96.7
Padial, 2005, Equatorial Guinea [41]	Total: 411, P. falciparum: 35 , P. ovale: 3, mixed: 1	CD 4000	72	98
Josephine, 2005, Malaysia [40]	Total: 889, P. vivax: 12, P. malariae: 3, P. falciparum: 1	CD 4000	100	100
de Langen, 2006, Namibia [42]	Total: 208, P. falciparum: 90	CD 3700 ≥1 depolarizing events	93	97
Hänscheid, 2008, Gabon [34]	Children, total: 368, P. falciparum: 152	CD 3000*** ≥1 depolarizing purple events Green-coded events	a) 96% b) 85%	a) 96% b) 96%
Hänscheid, 2009, Gabon [43]	Pregnant patients, total 685, P. falciparum: 86	CD 3000 ≥1 depolarizing events	86.8	78.5
Rathod, 2009, India [33]	Total: 523, P. falciparum :73, P. vivax: 62	CD 3200 ≥1 depolarizing events	62.2	25.3

^¶Index diagnostic test: abnormal depolarizing events in the side-scatter/depolarized side-scatter plot. ‡All studies use the instrument's diagonal separation line for eosinophils and neutrophils in the side-scatter/depolarized side-scatter plot, unless otherwise specified. ** CD: Cell-Dyn. †Imported malaria. ***For purple events, these were considered positive if present above a line traced at 5 pixels from the x axis. For green events, a special gate was created to identify haemozoin-laden granulocytes, with the intention to exclude eosinophils. In accordance with studies using flow cytometric cell sorting [27], the largest possible gate to the left and above the usual location of the eosinophil population was created which did not contain any eosinophils [34]. For this, CBC analyses from children without malaria or pseudoreticulocytosis were used [34]. ^§For the complete table with additional comments and reference diagnostic tests used in each study see Additional File 1.

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ISSN: 1475-2875

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