PfSHMTm immunofluorescence images showing localization in the apicoplast. (A) Early trophozoite showing no apicoplast PfSHMTm co-localization. (B) Early mitotic schizont with PfSHMTm fluorescence conforming closely to the 'C' shaped apicoplast. (C) Mitotic schizont with an elongating apicoplast, PfSHMTm fluorescence is concentrated within the distal portions of the organelle with little fluorescence in the medial section. (D) Post-mitotic schizont showing little spatial coincidence of PfSHMTm and the multiple apicoplasts (scale bars 3 μm, except (D). which is 2 μm). The associated table shows the percentage volume (V%) and material (M%) co-localization data for PfSHMTm (Sm) and acyl carrier protein (ACP) fluorescence.