Skip to main content

Table 2 Comparison of three DNA extraction methods for optimal recovery of DNA from unstained and stained TBFs and thin blood films.

From: Giemsa-stained thick blood films as a source of DNA for Plasmodium species-specific real-time PCR

  QIAGEN method Water method Chelex method
  Ct Pf Ct HBB Ct Pf Ct HBB Ct Pf Ct HBB
unstained TBF 38,21 33,92 0 44,69 0 0
stained TBF 38,92 32,25 0 0 0 0
unstained thin blood film 39,43 35,32 0 40,19 0 0
stained thin blood film 39,90 34,80 0 0 0 0
  1. Cycle threshold (Ct)-values were given for the detection of parasite DNA (Pf: Plasmodium falciparum) and human DNA by human beta globin (HBB) PCR.