Isolation of segmented stage irbc by increased irbc loading of buffer optimized HGMS columns. In this representative experiment, 3 × 109 rbc from an asynchronized culture containing app. 3% schizont irbc were applied to a HGMS column. Over 90% of segmented stage irbc are obtained, total irbc purity is >98%. Differential parasitemias were counted microscopically (1000 cells per sample).