Comparison of culture development after HGMS-depletion and sorbitol lysis. Bars represent the fraction of ring-stage irbc out of total irbc. Percentage of ring-stage irbc decrease over time from 97.7% for HGMS depletion and 98.9% for sorbitol lysis on day 0 (day of experiment and re-culture) to 79.3% for HGMS depletion and 84.8% for sorbitol lysis on day 6. No significant difference was observed between both methods. Data are from 5 independent experiments for HGMS-depletion and from 3 independent experiments for sorbitol lysis.