Optimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cells

Malaria Journal

Table 3 Comparison between existing irbc concentration and depletion methods and buffer optimized HGMS

Method	Application	Purity	Assay time	Cost per sample^A	Special equipment required
Percoll^®:	concentration	50-99%	1-1.5 h	2-3 USD	none
Sorbitol lysis:	depletion	97-99%	50 min	1-2 USD	none
Polymer-coated HGMS:	concentration	83-99%	45 min	10-15 USD	Magnet, HGMS columns
	depletion	NA	NA	-
Buffer optimized HGMS:	concentration	93-99%	45 min	6-8 USD	Magnet, HGMS columns
	depletion	97-98%	50 min

^A For concentration methods cost was estimated for isolation of 10⁷ irbc. assuming 95% purities of separations. Depletion cost refers to depletion of 2 × 10⁷ irbc from cultures.

ISSN: 1475-2875